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pampk α1 thr 172 (Novus Biologicals)

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Novus Biologicals pampk α1 thr 172

Low lactate level accompanies AMPK activation and limits mitochondrial biogenesis and metabolic cofactor pools. A, l-lactate levels in 3-, 6-, and 10-month-old D2G and D2 ONs (n = 8 ONs/group). B, Ratio of <t>pAMPK</t> to AMPK protein in 3-, 6-, and 10-month-old D2G and D2 ONs (n = 8 ONs/group). C, D, Phosphorylated AMPK immunofluorescence (magenta) and GFAP (green) micrographs (C) of human control and glaucoma ONs (n = 4 sections/ON, 2 ONs/group); and 3-month-old D2, and 10-month-old D2G and D2 mice (n = 3 sections/ON, 6 ONs/group; D). Arrows indicate colocalization of pAMPK and GFAP. E, F, Percentage of mean fluorescence intensity in the ROI for pAMPK 3-month-old D2, and 10-month-old D2G and D2 mice (E) and humans (F). G–I, Analyses of NAD+/NADH, CK activity, and PGC1-α levels in 3-, 6-, and 10-month-old D2G and D2 mice. G, NAD+ normalized to NADH levels (n = 6 ONs/group). See Figure 3-1. H, Creatine kinase activity normalized to total protein (n = 6 ONs/group). I, PGC1-α protein levels normalized to total protein levels and then to 3-month-old D2G protein levels (n = 8 ONs/group). All values are presented as the mean ± SEM, one-way ANOVA and Tukey's post hoc test. A, F(5,42) = 22.04, *p = 0.0124; B, F(5,42) = 35.51, **p = 0.0032, ***p = 0.0001; E, F(2,45) = 208.4, ***p = 0.0001; F, t(14) = 14.79, ***p = 0.0001, two-tailed unpaired t test; G, F(5,30) = 5.061, *p = 0.0012; H, F(5,48) = 38.28, **p = 0.0025; I F(5,42) = 5.43, *p = 0.0231. Scale bar, C, D, 20 μm.

Pampk α1 Thr 172, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pampk+%CE%B11+thr+172/p+ampk%CE%B1++thr172++rabbit+monoclonal+antibody/pmc05977447-18-0-14
Average 90 stars, based on 1 article reviews

pampk α1 thr 172 - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Structural and Functional Rescue of Chronic Metabolically Stressed Optic Nerves through Respiration"

Article Title: Structural and Functional Rescue of Chronic Metabolically Stressed Optic Nerves through Respiration

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.3652-17.2018

Figure Legend Snippet: Low lactate level accompanies AMPK activation and limits mitochondrial biogenesis and metabolic cofactor pools. A, l-lactate levels in 3-, 6-, and 10-month-old D2G and D2 ONs (n = 8 ONs/group). B, Ratio of pAMPK to AMPK protein in 3-, 6-, and 10-month-old D2G and D2 ONs (n = 8 ONs/group). C, D, Phosphorylated AMPK immunofluorescence (magenta) and GFAP (green) micrographs (C) of human control and glaucoma ONs (n = 4 sections/ON, 2 ONs/group); and 3-month-old D2, and 10-month-old D2G and D2 mice (n = 3 sections/ON, 6 ONs/group; D). Arrows indicate colocalization of pAMPK and GFAP. E, F, Percentage of mean fluorescence intensity in the ROI for pAMPK 3-month-old D2, and 10-month-old D2G and D2 mice (E) and humans (F). G–I, Analyses of NAD+/NADH, CK activity, and PGC1-α levels in 3-, 6-, and 10-month-old D2G and D2 mice. G, NAD+ normalized to NADH levels (n = 6 ONs/group). See Figure 3-1. H, Creatine kinase activity normalized to total protein (n = 6 ONs/group). I, PGC1-α protein levels normalized to total protein levels and then to 3-month-old D2G protein levels (n = 8 ONs/group). All values are presented as the mean ± SEM, one-way ANOVA and Tukey's post hoc test. A, F(5,42) = 22.04, *p = 0.0124; B, F(5,42) = 35.51, **p = 0.0032, ***p = 0.0001; E, F(2,45) = 208.4, ***p = 0.0001; F, t(14) = 14.79, ***p = 0.0001, two-tailed unpaired t test; G, F(5,30) = 5.061, *p = 0.0012; H, F(5,48) = 38.28, **p = 0.0025; I F(5,42) = 5.43, *p = 0.0231. Scale bar, C, D, 20 μm.

Techniques Used: Activation Assay, Immunofluorescence, Control, Fluorescence, Activity Assay, Two Tailed Test

$Monocarboxylate transport changes and AMPK activation occur with acute glaucoma injury. A, CTB (green) after intraocular injection from retina to SC of control and bead injected Mito-CFP mice. Dark regions of the superficial, retinorecipient areas of the SC indicate lack of axon transport from the retina. See Figure 4-1. B, Percentage area fraction of CTB transport in SC (n = 10 per group). C, RGCs immunolabeled for the RGC-specific antigen RBPMS in control and bead-injected Mito-CFP mice. D, Quantification of RGC density in control and bead-injected mice (n = 16 control mice; n = 14 bead-injected mice). E–G, Protein analysis by capillary electrophoresis of pAMPK/AMPK (E), MCT1 (F), and MCT2 (G). Proteins normalized to total protein (n = 6 ONs/group). All values are presented as the mean ± SEM, *two-tailed unpaired t test. B, t(16) = 15.07, ***p = 0.0001; D, t(27) = 5.412, ***p = 0.0001; E, t(10) = 4.165, ***p = 0.0019; F, t(10) = 3.114, *p = 0.0110; G, t(10) = 6.536, **p = 0.00110. Scale bars: A, 100 μm; C, 50 μm.$
Figure Legend Snippet: Monocarboxylate transport changes and AMPK activation occur with acute glaucoma injury. A, CTB (green) after intraocular injection from retina to SC of control and bead injected Mito-CFP mice. Dark regions of the superficial, retinorecipient areas of the SC indicate lack of axon transport from the retina. See Figure 4-1. B, Percentage area fraction of CTB transport in SC (n = 10 per group). C, RGCs immunolabeled for the RGC-specific antigen RBPMS in control and bead-injected Mito-CFP mice. D, Quantification of RGC density in control and bead-injected mice (n = 16 control mice; n = 14 bead-injected mice). E–G, Protein analysis by capillary electrophoresis of pAMPK/AMPK (E), MCT1 (F), and MCT2 (G). Proteins normalized to total protein (n = 6 ONs/group). All values are presented as the mean ± SEM, *two-tailed unpaired t test. B, t(16) = 15.07, ***p = 0.0001; D, t(27) = 5.412, ***p = 0.0001; E, t(10) = 4.165, ***p = 0.0019; F, t(10) = 3.114, *p = 0.0110; G, t(10) = 6.536, **p = 0.00110. Scale bars: A, 100 μm; C, 50 μm.

Techniques Used: Activation Assay, Injection, Control, Immunolabeling, Electrophoresis, Two Tailed Test

Figure Legend Snippet: List of antibodies used for IHC and capillary electrophoresis analyses

Techniques Used: Electrophoresis

Immunohistochemistry:

Article Title: Structural and Functional Rescue of Chronic Metabolically Stressed Optic Nerves through Respiration
Article Snippet: Phospho-AKT Ser 473 , 1:50 for Wes , AB_329825 , Cell Signaling Technology , Rabbit. .. pAMPK α1 Thr 172 , 1:100 for IHC, 1:50 for Wes , AB_11032916 , Novus Biologicals , Rabbit. .. p70S6K , 1:50 for Wes , AB_331676 , Cell Signaling Technology , Rabbit.

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Journal: The Journal of Neuroscience

Article Title: Structural and Functional Rescue of Chronic Metabolically Stressed Optic Nerves through Respiration

doi: 10.1523/JNEUROSCI.3652-17.2018

Figure Lengend Snippet: Low lactate level accompanies AMPK activation and limits mitochondrial biogenesis and metabolic cofactor pools. A, l-lactate levels in 3-, 6-, and 10-month-old D2G and D2 ONs (n = 8 ONs/group). B, Ratio of pAMPK to AMPK protein in 3-, 6-, and 10-month-old D2G and D2 ONs (n = 8 ONs/group). C, D, Phosphorylated AMPK immunofluorescence (magenta) and GFAP (green) micrographs (C) of human control and glaucoma ONs (n = 4 sections/ON, 2 ONs/group); and 3-month-old D2, and 10-month-old D2G and D2 mice (n = 3 sections/ON, 6 ONs/group; D). Arrows indicate colocalization of pAMPK and GFAP. E, F, Percentage of mean fluorescence intensity in the ROI for pAMPK 3-month-old D2, and 10-month-old D2G and D2 mice (E) and humans (F). G–I, Analyses of NAD+/NADH, CK activity, and PGC1-α levels in 3-, 6-, and 10-month-old D2G and D2 mice. G, NAD+ normalized to NADH levels (n = 6 ONs/group). See Figure 3-1. H, Creatine kinase activity normalized to total protein (n = 6 ONs/group). I, PGC1-α protein levels normalized to total protein levels and then to 3-month-old D2G protein levels (n = 8 ONs/group). All values are presented as the mean ± SEM, one-way ANOVA and Tukey's post hoc test. A, F(5,42) = 22.04, *p = 0.0124; B, F(5,42) = 35.51, **p = 0.0032, ***p = 0.0001; E, F(2,45) = 208.4, ***p = 0.0001; F, t(14) = 14.79, ***p = 0.0001, two-tailed unpaired t test; G, F(5,30) = 5.061, *p = 0.0012; H, F(5,48) = 38.28, **p = 0.0025; I F(5,42) = 5.43, *p = 0.0231. Scale bar, C, D, 20 μm.

Article Snippet: pAMPK α1 Thr 172 , 1:100 for IHC, 1:50 for Wes , AB_11032916 , Novus Biologicals , Rabbit.

Techniques: Activation Assay, Immunofluorescence, Control, Fluorescence, Activity Assay, Two Tailed Test

Journal: The Journal of Neuroscience

Article Title: Structural and Functional Rescue of Chronic Metabolically Stressed Optic Nerves through Respiration

doi: 10.1523/JNEUROSCI.3652-17.2018

Figure Lengend Snippet: Monocarboxylate transport changes and AMPK activation occur with acute glaucoma injury. A, CTB (green) after intraocular injection from retina to SC of control and bead injected Mito-CFP mice. Dark regions of the superficial, retinorecipient areas of the SC indicate lack of axon transport from the retina. See Figure 4-1. B, Percentage area fraction of CTB transport in SC (n = 10 per group). C, RGCs immunolabeled for the RGC-specific antigen RBPMS in control and bead-injected Mito-CFP mice. D, Quantification of RGC density in control and bead-injected mice (n = 16 control mice; n = 14 bead-injected mice). E–G, Protein analysis by capillary electrophoresis of pAMPK/AMPK (E), MCT1 (F), and MCT2 (G). Proteins normalized to total protein (n = 6 ONs/group). All values are presented as the mean ± SEM, *two-tailed unpaired t test. B, t(16) = 15.07, ***p = 0.0001; D, t(27) = 5.412, ***p = 0.0001; E, t(10) = 4.165, ***p = 0.0019; F, t(10) = 3.114, *p = 0.0110; G, t(10) = 6.536, **p = 0.00110. Scale bars: A, 100 μm; C, 50 μm.

Article Snippet: pAMPK α1 Thr 172 , 1:100 for IHC, 1:50 for Wes , AB_11032916 , Novus Biologicals , Rabbit.

Techniques: Activation Assay, Injection, Control, Immunolabeling, Electrophoresis, Two Tailed Test

Journal: The Journal of Neuroscience

Article Title: Structural and Functional Rescue of Chronic Metabolically Stressed Optic Nerves through Respiration

doi: 10.1523/JNEUROSCI.3652-17.2018

Figure Lengend Snippet: List of antibodies used for IHC and capillary electrophoresis analyses

Article Snippet: pAMPK α1 Thr 172 , 1:100 for IHC, 1:50 for Wes , AB_11032916 , Novus Biologicals , Rabbit.

Techniques: Electrophoresis

Journal: Journal of neuroscience research

Article Title: Hindbrain A2 Noradrenergic Neuron Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation, Upstream Kinase/Phosphorylase Protein Expression, and Receptivity to Hormone and Fuel Reporters of Short-Term Food Deprivation are Regulated by Estradiol

doi: 10.1002/jnr.23892

Figure Lengend Snippet: Description of Primary Antibodies Used

Article Snippet: ; rabbit; sc-25792; Lot no. 1009; AB_216946 0.4 ug/mL pAMPK Phosphorylated Thr 172 of human AMPK α1/2 Santa Cruz Biotechnol.

Techniques: Concentration Assay

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pampk α1 thr 172 (Novus Biologicals)

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